منابع مشابه
Hapten-sandwich Labeling
A hapten-sandwich procedure has been developed for specific labeling of cell surface antigens for fluorescence or electron microscopy. Haptens are azo-coupled to immunoglobulins specific for a cell surface antigen; the hapten-modified cell-bound antibodies can then be visualized by adding fluorescent antihapten antibody, or by adding antihapten antibody followed by hapten-modified markers for e...
متن کاملHapten-sandwich labeling. II. Immunospecific attachment of cell surface markers suitable for scanning electron microscopy
A hapten-sandwich procedure has been used for immunospecific labeling of cell surface antigens with markers visible by scanning electron microscopy. Antihapten antibody was used to link hapten-modified tobacco mosaic virus, bushy stunt virus, or hemocyanin to hapten-modified human erythrocytes. The antihapten antibody bridge was also used to link the hapten-virus marker to hapten-modified antib...
متن کاملHapten-syngeneic and Hapten-allogeneic Determinants*
A growing n u m b e r of immunolog ica l responses h a v e been shown to be unde r genet ic control. In genera l , these responses have been l inked to the major h is tocompat ib i l i ty complex (MHC),I and it appea r s t h a t i t is the gene products of the MHC which are cri t ical for the recogni t ion of self and not -se l f (1). Such MHC-l inked responses include the genera t ion and expr...
متن کاملExtrinsic Cotton effects in hapten--carrier and hapten--antibody interactions.
Two homogeneous univalent hapten-protein conjugates, prepared by the covalent attachment of a single 2,4-dinitrophenyl (DNP-) or 2,4,6-trinitrophenyl (TNP-) side chain to the cysteine-SH in the active site of the enzyme papain, have been found to exhibit large Cotton effects in the wavelength region of the absorption bands of the DNP or TNP groups. This indicates that the DNP or TNP groups are ...
متن کاملHapten-carrier relationships.
The ability of guinea pigs to recognize poly-I,-lysine (PLL), and hapten conjugates of PLL (H-PLL) as antigens is under the control of a single autosomal dominant gene which we will refer to as the PLL gene (1-3). Responder animals carrying this gene form specific antihapten antibodies and display delayed hypersensitivity reactions after immunization with H-PLL conjugates such as 2,4-dinitrophe...
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ژورنال
عنوان ژورنال: Journal of Experimental Medicine
سال: 1974
ISSN: 1540-9538,0022-1007
DOI: 10.1084/jem.140.2.523